| 대한안과학회 학술대회 발표 연제 초록 |
| 외안 F-004 |
| 전기방사상 섬유를 이용하여 구성한 3D scaffold에서 배양한 골수유래 조혈모세포의 secretome의 특성 |
가톨릭대학교 의과대학 (1), 가천대학교 화공생명공학화 (2), Stanford University (3) |
| 나경선(1), 이현종(2), David Myung(3) |
목적 : The therapeutic effects of secreted factors (secretome) produced by bone marrow-derived human mesenchymal stem cells (MSCs) were evaluated as a function of their growth in 2D culture conditions and on 3D electrospun fiber scaffolds. 방법 : Electrospun fiber scaffolds composed of polycaprolactone and gelatin were fabricated to provide a 3D microenvironment for MSCs.The secretome produced by the MSCs cultured on 3D fiber matrices versus 2D culture dishes were analyzed. 결과 : Concentrations of factors such as HGF and ICAM-1 were increased over 5 times in 3D cultures compared to 2D cultures. Scratch assays showed 95% closure at 48 hr when CFCs were co-cultured with MSCs seeded on fibers, while the control group only exhibited 50% closure at 48 hr. MSCs seeded on fibers promoted faster epithelialization and less scarring.Immunostaining showed that expression of alpha-SMA was lower in corneas treated with MSCs seeded on fibers, suggesting suppression of myofibroblastic transformation. 결론 : MSCs cultured on electrospun fibers facilitate wound healing in CFCs and on explanted corneas through differential secretome profiles compared to MSCs cultured on 2D substrates. |