| 대한안과학회 학술대회 발표 연제 초록 |
| 외안 F-003 |
| 마우스에서 Fucosyltransferase 1 유전자 제거가 정상 및 건조 자극 하 환경에서 면역 불균형 및 각막 혼탁 발생에 미치는 영향 분석 |
(1)서울대학교 의과대학 안과학교실 (2)서울대학교병원 의생명연구원 인공안구센터 안면역재생연구실 (3)서울대학교 의과대학 피부과학교실 |
| 김경우(1,2), 이현주(2), 고정화(2), 류진숙(2), 김현지(2), 김준엽(2), 오장희(3), 정진호(3), 오주연(1,2) |
목적 : Alpha-1,2-fucosyltransferase 1 (FUT1) is known to mediate the synthesis of H antigen that is a precursor protein of ABO blood antigen. Although the mechanisms are still unclear, several studies have elucidated that the change of FUT1 expression might be associated with diverse biological phenomena including inflammation, tumor growth, metastasis and angiogenesis. In this study, we investigated the role of FUT1 in the ocular surface and immune system using FUT1 knock-out (KO) mice in steady state and under desiccating stress. 방법 : Phenotypical indices in ocular surface including corneal epithelial integrity, tear production, corneal opacity and conjunctival goblet cells were analyzed in FUT1-KO and wild type (WT) C58BL/6 mice in steady state with age and under desiccating injury to the ocular surface. Transcript levels of inflammatory cytokines were measured in the ocular surface and lacrimal glands. Regional and systemic immune responses were investigated in cervical lymph nodes (CLNs) by flow cytometry and in cultures of splenocytes by mixed lymphocyte reaction (MLR) and lipopolysaccharide (LPS) stimulation assays. 결과 : FUT1 deficiency induced corneal epithelial defects and opacity in steady state. Especially, corneal opacity was more prominent in FUT1-KO mice under desiccating stress. Ocular surface inflammation and type 1 helper T cells in CLNs increased in FUT1-KO mice, compared to WT controls both in steady state and under desiccating stress. Similarly, splenocytes from FUT1-KO mice were highly CD4+ cell-stimulatory compared to the cells from WT mice and expressed higher level of interleukin-6. 결론 : FUT1 deficiency induces ocular surface inflammation, corneal opacification and immune dysregulation in mice both in the steady state and under desiccating stress. |