| 대한안과학회 학술대회 발표 연제 초록 |
| 백굴 F-009 |
| 방수 내 특이 바이오마커 감지 시 활성화되는 형광 센서 탑재 인공수정체 개발 |
연세대학교 의과대학 안과학교실 시기능개발연구소(1), 국민건강보험 일산병원 안과(2), 연세대학교 공과대학 화학생명공학과학교실 나노바이오시스템연구소(3) |
| 지용우(1,2), 신무광(3), 문채은(1,2), 이효(3), 김승환(1,2), 함승주(3), 이형근(1) |
목적 : The eye is an extension of the central nervous system (CNS) which is not encased in the skull, thus it allows for non-invasive visualization of early changes in CNS disorders beyond eye diseases. Due to recent advances in biosensing technology, there is a growing interest in the development of implantable devices such as the intraocular lens (IOL) for specific medical uses such as intraocular monitoring. Thus, we developed a novel biomarker-activatable peptide-conjugated hydrogel-based fluorogenic IOL sensing system. 방법 : After PEGDAAm was synthesized, cell viability test of sensor materials was done using the corneal endothelial cell line. Conjugation of peptide-probes inside the hydrogel were performed following protein diffusion test. As a proof of concept, matrix-metalloproteinase(MMP)-9 was selected as a target in AH. PHEMA-based IOL was prepared using 3D-printing and then the hydrogel sensor was attached to IOL to complete the FIOL. Under cataract surgery, the FIOL was implanted successfully in vivo. The fluorescence of FIOL was observed with a slit lamp after MMP-9 injection into the anterior chamber. 결과 : PEGDAAm was selected as a biocompatible hydrogel material, showing enhanced chemical stability not only in acidic and basic buffers but also in BSS. Hydrogel concentration was optimized to control the mesh size to allow biomarker penetration but not to allow other cells or proteins to diffuse. MMP-9 specifically activatable peptide-probe-conjugated fluorogenic PEGDAAm-co-PAA hydrogel sensor was developed and its LOD was analyzed. Finally, the clinical applicability of FIOL through long-term application in live rabbit eyes was demonstrated. 결론 : The biostable label-free MMP-9 hydrogel sensor attached IOL was the first to demonstrate not only hydrogel based macromolecular enzymatic biomarker sensing ability, but also potential clinical applications in vivo for long-term examination. This newly developed sensing system offers a promising approach for IOL-based liquid biopsy using intraocular fluids as clinical specimens. |