| 발표일자: |
2019년 11월 1일(금)~3(일) |
| 발표번호: |
P(e-poster)-161 |
| 발표장소: |
B3 Parking Area
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| 3차원 바이오 프린팅을 이용해 간략화 한 망막골격에서 실험한 당뇨병성 망막병증 |
| (1)경북대학교 의과대학 안과학 교실,(2)경북대학교병원 생명의학연구원 |
| 이성택(1), 엄희동(1), 윤동희(1), 이경필(2), 정성숙(2), 박한상(1), 박동호(1), 신재필(1), 김홍균(1) |
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목적 : The main role of Müller cells is to maintain the 3-dimensional (D) structural and physiological stability of retina. However, Müller cells usually were cultured in a cell culture dish (2D system). These are traditional experimental methods that disregard the role of the Müller cell, which play an important role in the 3D structure of the retina. In this study, we developed a novel 3D Müller biomimetic simplified retinal scaffolds using 3D bio-printer and in vitro evaluation of simulated diabetic retinopathy using 3D Müller biomimetic simplified retinal scaffolds.
방법 : In order to induce diabetic retinopathy, high-glucose (HG) and Advanced Glycation end product (AGE) – Bovine serum albumin (BSA) treatment were compared in both 2D system and 3D Müller biomimetic simplified retinal scaffolds.
결과 : As both methods were processed, expression of Glutamine synthetase (GS) and Kir 4.1 decreased in both 2D system and 3D Müller biomimetic simplified retinal scaffolds, and expression of Glial fibrillary acidic protein (GFAP) and Vimentin increased. In addition, in HG and AGE-BSA, the secretion of VEGF-A and inflammatory cytokines as well as RAGE, which induces inflammatory responses, were significantly increased in 3D Müller biomimetic simplified retinal scaffolds than in 2D.
결론 : As a result, Müller cells cultured in 3D Müller biomimetic simplified retinal scaffolds are similar to those of the actual retinal Müller cells and functionally create a better environment condition than 2D. It is expected that could be the basis for implementing 3D models of the retina and a possibility of an in vitro drug screening system related to DR.
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