| 발표일자: |
2019년 11월 1일(금)~3(일) |
| 발표번호: |
P(e-poster)-364 |
| 발표장소: |
B3 Parking Area
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| 각막 내피세포 기능 부전과 연관된 방수 단백질 생물학적 기전 |
| 성효진(1,2), 이승재(1,2), 류선영(1,2), 문채은(1,2), 정재훈(1,2), 지용우(1,2) |
| 연세대학교 의과대학 안과학교실 시기능개발연구소(1), 국민건강보험 일산병원 안과(2) |
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목적 : To investigate biologic mechanism of aqueous humor (AH) biomarkers associated with corneal endothelial cell (CEC) function
방법 : We collected AH from 5 patients with CECD during descemet membrane endothelial keratoplasty (DMEK) and also did intraopertively from 5 patients with only cataract as an age-/sex-matched control group. CEC density was measured by using specular microscope and thickness was by ultrasound pachmeter. We used LC-MS/MS with high-pH RPLC fractionation for global proteome profiling. Proteins with expression greater than ±2-fold change in LFQ intensity were classified to differentially expressed proteins (DEPs). Bioinformatics analyses was performed with GO-BP and PPI network.
결과 : 833 proteins and 839 proteins were identified from AH in control and CECD group, respectively. 51 DEPs were up-regulated (UP-DEP) and 78 were down-regulated (DN-DEP) in AH of CECD. Those were enriched in the extracellular region, membrane-bounded vesicle, and secretory granule. UP-DEPs were mainly enriched in proteolysis, catalytic activity and immune-associated processes, while DN-DEPs in signaling and development process, cellular metabolism and nervous system-related processes. Interestingly, carbonic anhydrase 2, as known CEC pump protein, significantly decreased in CECD (p <0.001).
결론 : In the present study, we proposed AH biomarkers and their functional processes regarding corneal endothelial dysfunction. Our findings would be meaningful translational approach using an integrated proteomic analysis with liquid biopsy.
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