| 발표일자: |
2019년 11월 1일(금)~3(일) |
| 발표번호: |
P(e-poster)-082 |
| 발표장소: |
B3 Parking Area
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| 수정된 유리체강내 주사법으로 망막으로의 AAV 형질 도입 개선 |
| 1.순천향대학교 대학원 의생명과학과(협동)
2.순천향대학교 부천병원 망막 및 황반 변성 중개연구소
3. 순천향대학교 부천병원 안과
4. 순천향대학교 천안병원 안과학교실
5. 순천향대학교 부천병원 안과학교실 |
| 산자르마드라히모브1,2,양진영1,2,안동혁2,한정우3,박태관1,2,3,4,5 |
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목적 : Intravitreal (IVT) injection method is a choice when targeting the inner retina for gene therapy. However, the transduction efficiency of AAV vectors administered by IVT route is usually low and may be affected by several factors.
방법 : To improve the transduction efficiency, we developed a novel illuminated long-needle attached injection system and injected AAV2-CMV-eGFP in front of the retina in the right eye of rabbits. With the conventional needle (30G, 13mm), AAV2-CMV-eGFP was also injected to rabbit eyes. Left eye of rabbits in both groups received 0.1ml of BSS and served as control. To compare transduction efficiency and side effects between groups, ophthalmological examinations and pro-inflammatory cytokines in the aqueous humor were assessed during 1 month. Retinal tissues were used for IHC.
결과 : In the ophthalmological examinations, no significant inflammatory signs were detected in both groups, except transient, mild hyperemia. In the whole mount and the vertically sectioned tissues of the test group, significantly increased GFP expression was detected at the ganglion cell and the inner nuclear layers at 1month (p<0.01). However, glial activation detected with GFAP/Iba-1 antibody were no differences between groups. Differences of Interleukin-6 and -8 expressions in aqueous humor were not observed either.
결론 : These results suggest that modified deep intravitreal injection in front of the retina would be safe and efficiently transduce AAV2 vectors into the retina of large animals and considered as a potential method for use in clinical trials.
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