| 발표일자: |
2019년 11월 1일(금)~3(일) |
| 발표번호: |
P(e-poster)-317 |
| 발표장소: |
B3 Parking Area
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| 건성안 환자의 안표면 염증 측정에 있어서 결막 염색의 효용성 |
| 충주의료원 안과(1), 서일대학교 생명화공학과(2), 가톨릭대학교 의과대학 서울성모병원 안센터(3), 밀란대학교 루이지 사코병원(4) |
| 양순원(1), 이현정(2), 김다영(3), 신수정(3), 스테파노 바라비노(4), 정소향(3) |
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목적 : To investigate the expression levels of inflammatory cytokines in the conjunctival epithelium and correlations with clinical parameters in dry eye disease(DED).
방법 : This study evaluated 28 patients with Sjögren syndrome(SS) DED,28 patients with non-SS DED and 10 controls. The messenger ribonucleic acid(mRNA) expression of inflammatory cytokines such as tumor necrosis factor-α,interleukin(IL)-1β,IL-6,interferon(IFN)-γ,IL-17 and matrix metalloproteinase 9(MMP9) from conjunctival epithelium was investigated by real-time polymerase chain reaction. Protein expression was confirmed by immunofluorescence staining. Correlations were evaluated between the mRNA expression of inflammatory cytokines and clinical DED parameters.
결과 : Patients with non-SS DED expressed significantly more IFN-γ,IL-6 and MMP9 genes in the conjunctival epithelium than the controls and all cytokine expression was significantly higher in SS DED patients than in the controls. Conjunctival staining scores significantly correlated with the expression of IFN-γ,IL-6,IL-17 and MMP9 in both DED groups. Correlation coefficients of all cytokines were much higher in SS DED compared to non-SS DED. Corneal staining scores showed positive correlations with IFN-γ,IL-17,MMP9 and correlation coefficients were lower than those of conjunctival staining scores.
결론 : Conjunctival staining scores may be useful to measure ocular surface inflammation in SS and non-SS DED.
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