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목적 : To evaluate the anti-inflammatory effects of angiogenin (ANG) in an endotoxin-induced anterior uveitis (EIU) rat model and investigate the pathways involved.
방법 : EIU rats were treated with three types of eye drops: balanced salt solution (BSS), mutant ANG (mANG), or wild type ANG (ANG) for three days. The integrity of the blood-aqueous barrier was evaluated by determining the infiltrating cell count and protein concentrations in aqueous humor. Histopathology was also studied. Western blot and qRT-PCR of aqueous humor and ocular tissue were performed to analyze the mRNA levels of various inflammatory cytokines and transcription factors.
결과 : EIU rats treated with ANG had decreased inflammatory cells and protein concentrations in the anterior chamber. Compared to BSS- and mANG-treated eyes, qRT-PCR results of ANG-treated eyes showed reduced expression of IL-1β, IL-8, TNF-α, and MyD88, while the expression of IL-4 and Il-10 was increased. Western blots of ANG-treated eyes showed decreased expression of IL-6, iNOS, IL-1β, TNF-α, and phosphorylated NF-kB and increased expression of IL-10.
결론 : ANG seems to effectively reduce immune-mediated inflammation in the EIU rat model by reducing the expression of pro-inflammatory cytokines, while increasing the expression of anti-inflammatory cytokines through pathways related to Myd88 and NFkB. Therefore, ANG shows potential for effectively suppressing immune-inflammatory responses in vivo.
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