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목적 : Phosphodiesterase (PDE) inhibitors increase matrix metalloproteinase (MMP) activity by inhibiting re-uptake of adenosine and may potentiate nitric oxide (NO) activity. This study performed to investigate mechanisms of the effects of PDE inhibitors on the trabecular outflow in cultured human trabecular meshwork cells (HTMC).
방법 : Primary HTMC cultures were exposed to 0, 20, and 50 μM dipyridamole (DPD) or theophylline (TPN). The permeability through HTMC monolayer was assessed using carboxyfluorescein. The production of NO was assessed with the Griess assay and the levels of MMP-2 measured by Western blotting.
결과 : DPD increased permeability significantly accompanied with increased nitrite concentration and MMP-2 activity (all p<0.05). TPN increased nitrite but did not affect the permeability or MMP-2 activity significantly (p>0.05). When treated with DPD and TPN together, both permeability and nitrite production were increased, but the MMP-2 activity showed no difference compared to exposing DPD alone (p>0.05).
결론 : DPD increased trabecular permeability accompanied with increased nitrite production and MMP-2 activity. PDE inhibitor may increase trabecular outflow by increasing MMP-2 activity and by potentiating NO activity through cGMP.
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