| International FP-013 |
| Role of Wnt/β-catenin Signaling in Adipogenesis of Orbital fibroblast from Graves’ orbitopathy |
| Department of Ophthalmology, Soonchunhyang University College of Medicine,
Soonchunhyang University Bucheon Hospital, Bucheon, Korea(1)
Department of Ophthalmology, Severance Hospital, The Institute of Vision Research, Yonsei University College of Medicine, Seoul, Korea(2) |
| Sang Earn Woo, M.D.(1), Sang Joon Jung, M.D.(1), Jesang Lee, M.D.(1), Tae Kwann Park, M.D., Ph.D.(1), Jin Sook Yoon, M.D., Ph.D.(2), Sun Young Jang, M.D., Ph.D.(1) |
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Purpose : To investigate the role of Wnt/β-catenin signaling in adipogenesisin an in vitro model of Graves’ orbitopathy (GO).
Methods : Orbital fat/connective tissues were obtained from GO and non-GO patients for primary orbital fibroblast (OF) cultures. We compared the expression levels of Wnt 5a, Wnt 10b, β-catenin, phosphoβ-catenin and cyclin D1 between GO and non-GO OF. During adipogenesis of GO OF, the expression levels of Wnt 5a, Wnt 10b, β-catenin and cyclin D1 was determined. The effects of stimulator and inhibitor of Wnt/β-catenin signaling on adipogenesis of GO OFwere investigated.
Results : Western blot analyses showed that the significant decrease of β-catenin and cyclin D1 and the significant increase of phosphoβ-catenin in OF from GO than in non-GO (P<0.05).Wnt 5a, Wnt 10b, β-catenin and cyclin D1 expressions are highest in OF (Day 0) and declines gradually after induction of differentiation. The expression levels of PPAR-γ, CEBP-α and CEBP-β were increased in Wnt/β-catenin inhibitor treated OFandgradually decreased in Wnt/β-catenin stimulator treated OF as a dose-dependent manner
Conclusion : These results indicate that Wnt/β-catenin signaling inhibits adipogenesis in OF from GO.Further studies are required to examine the potential of Wnt/β-catenin signaling as a target for therapeutic strategies.
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