| International FP-008 |
| Therapeutic effect of curcumin, a plant polyphenol extracted from Curcuma longae, in an in vitro model of Graves’ orbitopathy |
| Department of Ophthalmology, Severance Hospital, Institute of Vision Research, Yonsei University College of Medicine, Seoul, Korea(1) |
| Jihei Sara Lee, MD(1); Jinjoo Kim, B.Sc.(1); and Jin Sook Yoon, MD, Ph.D(1) |
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Purpose :
Recent studies have identified ant-inflammatory, anti-oxidant and anti-adipogenic properties of curcumin, a plant polyphenol extracted from Curcuma longae. We investigated whether non-toxic concentrations of curcumin have therapeutic effect in primary cultures of orbital fibroblasts from Graves’ orbitopathy(GO).
Methods : Orbital connective tissues were acquired from patients with GO and healthy controls and cultured to obtain orbital fibroblast. Fibroblasts were pre-treated with curcumin for 1 hour, and exposed to interleukin(IL)-1β, adipogenic differentiation medium, cigarette smoke extract(CSE), and H2O2. Release of inflammatory cytokines was quantified using quantitative real time PCR, enzyme-linked immunosorbent assay(ELISA), and western blot analysis. Adipogenic differentiation was identified using Oil-Red O staining. Reactive oxygen species(ROS) was measured using an oxidant-sensitive fluorescent probe.
Results : Treatment with curcumin resulted in decrease in IL-1β-induced synthesis of IL-6 and IL-8. Decrease in lipid droplets and expression of peroxisome proliferator activator gamma(PPARγ) and CCAAT-enhancer-binding proteins(C/EBP) α/β were noted in fibroblasts treated with curcumin during adipocyte differentiation. CSE- and H2O2-induced ROS synthesis was significantly lower in curcumin-treated fibroblasts compared to the control. Curcumin significantly countered activation of Erk, Akt, Jnk and NF-κB p65 proteins by IL-1β and elevated β-catenin translocation into nucleus during adipogenesis.
Conclusion : Curcumin inhibits pro-inflammatory cytokine production, ROS synthesis and adipogenesis in orbital fibroblasts of GO patients in vitro possibly by affecting multiple pro-inflammatory signaling molecules and β-catenin pathway. The results of the study support potential use of the curcumin in the treatment of GO.
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