| International FP-009 |
| The anti-inflammatory effect of Glucagon-like peptide-1 (GLP-1) agonist on peripapillary retinal nerve fiber layer and retinal nuclear layers in the diabetic mouse model |
| (1) Department of Ophthalmology and Visual Science, St. Vincent's Hospital, the Catholic University of Korea
(2) Department of Ophthalmology and Visual Science, St. Mary's Hospital, the Catholic University of Korea
(3) Clinical Research Center, St. Vincent's Hospital, the Catholic University of Korea
(4) Department of Endocrinology, St. Vincent's Hospital, the Catholic University of Korea |
| Yeon Woong Chung(1), Jae Hyung, Lee(2), Hyun Hui Joo(3), Ye-Jee Lee(4), Dong Hyun Jee(3), Seung-Hyun Ko(4), Jin A Choi(1) |
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Purpose : Glucagon-like peptide (GLP)-1 agonist has been known to reduce inflammatory process as well as to control diabetes mellitus (DM). We compared anti-inflammatory effects of peripapillary retinal nerve fiber, outer nuclear and inner nuclear layer in diabetes mice with intravenous GLP-1 agonist, insulin and untreated groups, and normal control group.
Methods : We divided diabetes mice into 3 groups; intravenous GLP-1 agonist (n = 8), insulin (n = 7) and untreated (n = 7). Control group was defined as wild type mice (n = 5). After 8 weeks later, we sacrificed these mice, and observed inflammatory markers (glial fibrillary acidic protein (GFAP) and glutathione synthetase (GS), and etc.) in eyeballs using Q-PCR. We divided peripapillary area into 2 sections; within 300 um from the optic nerve head (central optic nerve head area) and outside 300 um from the optic nerve head (peripheral optic nerve head area) and analyzed the thickness of retinal nerve fiber, outer nuclear and inner nuclear layers and immunohistochemistry (IHC) results of GFAP and thioredoxin interacting protein (TXNIP).
Results : The untreated mice group showed statistically increased inflammatory markers than the other groups. Of 2 treated groups, the GLP-1 agonist group revealed significantly decreased GFAP and GS levels than the insulin group. The GLP-1 agonist group showed statistically decreased retinal nerve fiber layer in the central optic nerve head area than the untreated group, but the insulin group did not showed statistically difference compared with the untreated group. In the IHC, the GLP-1 agonist group showed significantly decreased GFAP and TXNIP than the other groups.
Conclusion : In the diabetes mouse model, the GLP-1 agonist was superior in anti-inflammatory effect than the untreated and even conventional insulin treatment. In the future, the GLP-1 agonist may be a good candidate to replace insulin for reducing inflammatory process in diabetic retinopathy patients.
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