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목적 : To investigate the characteristics of human orbital fibroblasts (OFs) cultivated from intraconal, nasal, and central adipose tissues
방법 : Intraconal adipose tissues were obtained during orbital decompression surgery for severe proptosis in six patients with Graves’ orbitopathy (GO). Nasal and central adipose tissues were obtained during upper eyelid blepharoplasty in six patients with no history of GO. Human OFs were separately cultured from GO intraconal, non-GO nasal, non-GO central orbital adipose deposits. Expression of IGF-1 and TSH receptors were investigated using quantitative RT-PCR. Protein levels of IL-1β-induced inflammatory cytokines anand generated intracellular reactive oxygen species (ROS) were determined
결과 : IGF-1 and TSH receptor RNA expressions of GO intraconal OFs and non-GO nasal OFs were higher than non-GO central OFs and dermal fibroblasts. The expression of IL-1β protein induced the IL-6, IL-8, ICAM-1, and COX2 of GO intraconal OFs, and non-GO nasal OFs were higher than non-GO central OFs and dermal fibroblasts. Intracellular ROS generation in GO intraconal OFs and non-GO nasal OFs were higher than in non-GO central OFs and dermal fibroblasts, although the differences were not statistically significant.
결론 : Non-GO nasal OFs had similar characteristics to GO intraconal OFs. We recommend the use of nasal adipose tissue in order to culture OFs as a normal control involving in vitro experiments
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