| 백굴 F-001 |
| 빛간섭단층촬영 및 세극등을 이용한 세 가지 종류의 자극하에서 관찰되는 BL6/N 마우스의 수정체 혼탁 정도에 관한 연구 |
| 울산대학교 의과대학 안과학교실(1), 연세대학교 의과대학 안과학교실(2), 국가마우스감각기표현형분석사업단(3), 국립의료원(4), 서울대학교 수의과대학(5), 국가마우스표현형분석사업단(6) |
| 이훈(1,3), 김홍경(2,3), 신해솔(2,3), 한수정(2,3), 윤상철(2,3,4), 성제경(5,6), 서경률(2,3) |
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목적 : To investigate conditions which cause temporal lens opacity, we tested chemical and physical factors, such as anesthesia dose, eye dryness, and light intensity in anesthetized BL6/N mice
방법 : Mice were anesthetized with low (80%; tiletamine/zolazepam (32 mg/kg, intraperitoneal injection) and xylazine (8 mg/kg, intraperitoneal injection) or high (120%; tiletamine/zolazepam (48 mg/kg) and xylazine (12 mg/kg) doses of anesthetic and examined bilaterally every 5 min from 10 min of anesthesia to 30 min after anesthesia. Using OCT and slit-lamp biomicroscopy, we tested three different factors such as anesthesia dose, eye dryness, and light intensity to investigate relevant factors which cause temporal lens opacity and explored the reversibility of lens opacity in anesthetized BL6/N mice.
결과 : Levels of lens opacity were assessed and graded (from 0 to 5) by the standard classification system we developed for this study. Corneal dryness was significantly associated with the formation of temporal lens opacity. However, lens opacity caused by corneal dryness was not strongly influenced by anesthesia dose. Additionally, lens opacity was not strongly affected by light, suggesting that anesthesia dose and light intensity do not mainly influence the formation of lens opacity. Lens opacity in the dry ocular environment was temporary and did not become an irreversible type of cataract.
결론 : In the current study, we demonstrate that corneal dryness might be a relevant factor for the formation and progression of lens opacity in anesthetized mice. Thus, our results may help researchers to minimize the formation of lens opacity during eye phenotyping in mutant mice.
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