| 발표일자: |
2019년 4월 5일(금)~7(일) |
| 발표번호: |
P(e-poster)-264 |
| 발표장소: |
벡스코 전시장 1홀 내 |
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| The anti-angiogenic effect of anthocyanin oligomer in a mouse model of retinopathy of prematurity |
| Department of Ophthalmology, Incheon St. Mary’s Hospital, The Catholic University of Korea, Incheon, Korea |
| HaeYeon Yoon, Hyung Bin Hwang, Kui Dong Kang |
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Purpose : Anthocyanins belong to the group of phytochemicals known as flavonoids. Studies have shown that its oligomeric derivatives have higher activity than the monomeric from. The purpose of the present study was to determine the anti-angiogenic effect of anthocyanin oligomer(AO) in a mouse model of retinopathy of prematurity.
Methods : ICR neonatal mice were exposed to 75% oxygen from postnatal day (P)7 until P12 and returned to room air for five days (P12 to P17). Mice were subjected to daily intraperitoneal injection of AO (10 mg/kg, 50 mg/kg) and vehicle from P12 to P17. Retro-orbital injection of FITC-dextran was performed and retinal flat mounts were viewed by fluorescence microscopy. Central avascular area was quantified from the digital images in a masked fashion using image analysis software. Neovascular tufts were quantified by using SWIFT_NV and neovascular lumens were quantified from histologic section in a masked fashion. Immunohistochemistry and Western blot analysis were also performed to demonstrate the anti-angiogenic activity of this compound in vivo.
Results : In the retina of AO injected mouse (50mg/kg), the central non-perfusion area was significantly decreased compared to the vehicle injected group (1.98±0.42 mm2 vs 2.78±0.73 mm2, P<0.05). In vehicle-injected group, 29.43 ± 4.89% of the total retinal area was avascular, whereas the retinas of pups treated with high-dose (50 mg/kg) AO showed avascular retinal areas of 18.31 ±4.21% (P<0.05). High dose of AO also significantly reduced the number of vascular lumens in the histologic section. AO (50 mg/kg) significantly reduced the expression of VEGF, MMP-2, MMP-9, and angiotensin II compared to the vehicle group.
Conclusion : Our results show that AO possesses anti-angiogenic properties and can attenuates new vessel formation in the retina after systemic administration. These results implies that this compound can be considered as a candidate substance for therapeutic inhibition of retinal angiogenesis.
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