| 발표일자: |
2019년 4월 5일(금)~7(일) |
| 발표번호: |
P(e-poster)-243 |
| 발표장소: |
벡스코 전시장 1홀 내 |
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| 각막 배양 모델에서 알칼리 화상에 교차결합된 콜라겐 수송체 안에 캡슐화된 중간엽 줄기 세포의 효과 |
| 가톨릭대학교 의과대학 안과학교실 |
| 김주영, 하민지, 황웅주, 황호식, 김현승, 나경선 |
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목적 : Alkali burns of the cornea cause a profound inflammatory response. The optimal way to deliver bone marrow-derived human mesenchymal stem cells (MSCs) to the eye is still unknown. Here we investigate strategies to deliver hMSCs and their secreted factors to the corneal surface in a rabbit organ culture model after alkaline burn. The delivery methods are comprised of (1) hMSCs within collagen gels crosslinked by copper-free click chemistry, (2) hMSCs within collagen gels crosslinked by a multifunctional PEG-N-hydroxysuccinimide (NHS) linker and (3) hMSCs in culture media.
방법 : An ex vivo organ culture model using rabbit corneas were subject to an alkaline burn using 1M NaOH-soaked filter paper. The corneas were washed abundantly after the alkaline burn to a pH of 7. The following treatments were applied to the cornea surfaces: hMSCs within collagen gels crosslinked by copper free click chemistry, hMSCs within collagen gels crosslinked using four-arm PEG-NHS, and hMSCs in media. Fluorescein dye was used to track the closure of the epithelial wounds, and cryosections of the treated and untreated corneas were fixed and stained for alpha smooth muscle actin (alpha-SMA).
결과 : The corneas lost their transparency immediately after alkali burn. Corneas treated with hMSCs healed faster and were more transparent than no treatment controls at one week. Corneas treated with hMSCs encapsulated within collagen gels cross-linked by click chemistry exhibited a higher degree of transparency compared to treatment with cells encapsulated in collagen gels crosslinked by 4-arm PEG-NHS. Treatment with hMSCs within both types of crosslinked gels expressed less alpha-SMA compared to no treatment and hMSCs alone.
결론 : hMSCs and their secreted factors have the potential to heal severe alkaline burns of the cornea. In preliminary work in ex vivo rabbit corneas, we have shown that transparency after alkaline burn is especially improved with topical application of hMSCs encapsulated within collagen gels crosslinked by click chemistry. Further work is merited to ascertain the mechanisms by which these cross-linked gels foster improved therapeutic effects from hMSCs.
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