| 발표일자: |
2019년 4월 5일(금)~7(일) |
| 발표번호: |
P(e-poster)-198 |
| 발표장소: |
벡스코 전시장 1홀 내 |
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| Bioenergic nucleotide nanoparticle이 Na+/K+ 의존성 ATPase의 활성화와 각막내피세포의 생존율에 미치는 영향 |
| 1. 가톨릭대학교 의과대학 안과학교실, 가톨릭대학교 인천성모병원
2. 가톨릭대학교 생명과학 대학원
3. 가톨릭대학교 약학대학 |
| 박선경1, Ramsha Afzal2, 김용찬1, 임혜빈1, 강규동1, 강한창3, 황형빈1 |
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목적 : Sodium and Potassium dependent Adenosine triphosphate enzymes exist in the membrane of the corneal endothelial cell that control the corneal hydration through the pump function and maintain transparency of the cornea. However, the role of Adenosine triphosphate to generate bioenergy is limited due to its highly anionic nature that leads to repulsion by the cationic cellular membrane. Here, we introduced an ionic complexation between Ca2+ and ATP to form nanocomplexes (NCs) and demonstrated the hypothesis that this new form of ATP can increase the survival of corneal endothelial cells in vitro.
방법 : Bovine corneal endothelial cells (BCEN) were treated with growth inhibiting drugs like Staurosporine (ST), Ouabain (OU), and Hydrogen peroxide (H2O2). Along with that, ATP and Ca2+/ATP NCs were co-treated to observe the comparative viability of the cells in each group. Cell viability assay (MTT), Live/Dead cell staining and TUNEL assay were performed to test whether Ca2+/ATP NCs are more effective than pure form of ATP.
결과 : Co-treatment of BCEN cell with drugs (ST, OU, and H2O2) and Ca2+/ATP or ATP showed increase in viability compared to controls (untreated and drug treated) and Ca2+/ATP NCs was found more effective than ATP.
Live/Dead staining also indicated greater survival in Ca2+/ATP NCs than ATP and other inhibiting drugs. Furthermore, TUNEL assay also confirmed our hypothesis of Ca2+/ATP to be more effective for BCEN.
결론 : It is concluded from our data that Ca2+/ATP NCs increases the survival of corneal endothelial cells than pure form of ATP in vitro.
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