| 발표일자: |
2019년 4월 5일(금)~7(일) |
| 발표번호: |
P-013 |
| 발표장소: |
벡스코 전시장 1홀 내 |
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| 레이저 광응고술 후 인간 망막 색소 상피 세포 재생과정에서 mTOR signaling의 필수적 역할 연구 |
| 1) 순천향대학교 대학원 의생명과학과 (협동)
2) 순천향대학교 부천병원 망막 및 황반 변성 중개연구소
3) 순천향대학교 부속 부천병원 안과
4) 순천향대학교 의과대학 안과학교실 |
| 산자르마드라히모브(1,2), 양진영(1,2), 박하얀(2), 박태관(1,2,3,4) |
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목적 : This study assessed the role of mechanistic target of rapamycin (mTOR) pathway in the human adult retinal pigment epithelial (ARPE) cells response after laser photocoagulation (LP).
방법 : The effect of mTOR inhibition on ARPE-19 cell was investigated by rapamycin treatment after LP. Cell viability and proliferation were explored using MTT and EdU assays, respectively. The expression of mTOR related proteins and epithelial-mesenchymal transition (EMT) markers were verified by Western blot.
결과 : Rapamycin retarded the LP area recovery in a dose-dependent manner by the 120 h, while LP+DMSO vehicle-treated cells completely restored the lesion zone (P≤0,01). ARPE-19 cell viability significantly lower in LP + rapamycin 80 and 160 ng/ml treated cultures comparing to LP control at 120 h (P≤0,001). LP control group demonstrated significantly more proliferative cells compared to untreated cells at the 72 and 120 h, whereas EdU-positive cell numbers in cultures treated with rapamycin at concentrations of 80 and 160 ng/ml were similar to baseline values. (P≤0,01).
결론 : mTOR pathway activation is essential for regulation of the RPE cell migration and proliferation after LP. mTOR inhibition with rapamycin effectively blocks the migration and proliferation of the RPE cells. Our results demonstrate that mTOR has an important role in ARPE-19 cell as a regulator of cell behavior under stress conditions, suggesting that mTOR could be a promising therapeutic target for numerous retinal diseases.
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