| GL F-018 |
| Effect of Losartan on the CMV infection in human trabecular meshwork cells |
| 1. Department of Ophthalmology, College of Medicine, St. Vincent’s Hospital 2. Department of Microbiology, College of Medicine, The Catholic University of Korea 3. Research Institute of St. Vincent's Hospital, College of Medicine, Catholic University of Korea 4.Department of Ophthalmology, College of Medicine, Seoul St. Mary’s Hospital |
| Jin A Choi (1), Ju-Eun Kim (2), Ju Hyun-hee (1,3), Donghyun-Jee (1), Soon-Young Paik (3), Chan Kee Park (4) |
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목적 : Human cytomegalovirus (CMV) has been demonstrated as causes of acute, recurrent, and chronic hypertensive anterior uveitis. In hypertensive anterior uveitis, human trabecular meshwork (TM) cells are considered to be a focus of inflammation. We investigated the effects of losartan, an angiotensin II receptor antagonist, on the CMV infection in trabecular meshwork cells.
방법 : Human TM cells were infected with CMV AD169, and additionally treated with losartan, dexamethasone, ganciclovir, and TGF-β1. Viral DNA accumulation and host cell response were analyzed using real-time PCR. Levels of secreted TGF-β1 were measured by determining its concentration in conditioned medium using a commercially available sandwich enzyme-linked immunosorbent assay (ELISA) kits.
결과 : The amount of viral DNA was increased exponentially at 1-7 day post infection (dpi). Losartan treatment significantly decreased the CMV copy numbers at 7 dpi. CMV-infected TM cells showed significant increase in the concentration of TGF-β1 at 3,5, and 7 dpi. Additional treatment with dexamethasone and losartan significantly decreased the TGF-β1, whereas treatment with ganciclovir did not affect the TGF-β1 level. CMV-infected TM cells stimulated by TGF-β1 significantly increased the expression of α-SMA and CTGF, which were decreased by the additional treatment with losartan.
결론 : Losartan inhibited the expressions of TGF-β1 and fibrogenic molecules in human trabecular meshwork cells. Thus, losartan appears to have the potential to decrease trabecular meshwork fibrosis in patients with viral anterior uveitis.
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