| CO F-018 |
| Bio-Orthogonally Crosslinked, In Situ Forming Corneal Stromal Tissue Substitute |
| 1 Catholic University of Korea, School of Mecinine, Department of Ophthalmology
2 Gachon University, Department of Chemical and Biological Engineering
3 Byers Eye Institute at Stanford University School of Medicine |
| Kyung-Sun Na(1), Hyun Jong Lee (2), Hyun Seung Kim (1), David Myung (3) |
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목적 : In this study, an in situ forming corneal stromal substitute based on collagen type I crosslinked by bio-orthogonal strain-promoted azide–alkyne cycloaddition (SPAAC) is presented.
방법 : Azide-PEG-collagen and DBCO-collagen were mixed as 1:1 to fabricate collagen gel crosslinked by click chemistry, and the mixed collagen gels were incubated for 10 – 15 min at 37 ºC for completion of gelation. For cell encapsulation, DBCO-collagen was added to the cell suspension in a 1:1 volumetric ratio.
결과 : Encapsulated corneal keratocytes grow within the SPAAC-crosslinked gels and corneal keratinocytes are supported on top of the gel surfaces. SPAACcrosslinked gels support more favorable and stable keratinocyte morphology on their surface compared to non-crosslinked gels likely as a result of more optimal substrate stiffness, gel integrity, and resistance to degradation.
SPAAC-crosslinked collagen gels with and without encapsulated keratocytes applied to rabbit corneas in an organ culture model after keratectomy exhibit surface epithelialization with ultilayered morphology.
결론 : The novel in situ forming gel is a promising candidate for lamellar and defect reconstruction of
corneal stromal tissue.
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