| RE F-005 |
| Involvement of mTORC1 and mTORC2 complexes in laser-induced choroidal neovascularization (CNV) in Mice. |
| (1) Department of Interdisciplinary Program in Biomedical Science, Soonchunhyang Graduate School, Bucheon Hospital, Bucheon, South Korea
(2) Laboratory for Translational Research on Retinal and Macular Degeneration, Soonchunhyang University Hospital Bucheon, Bucheon, South Korea
(3) Department of Ophthalmology, Soonchunhyang University Hospital Bucheon, Bucheon, South Korea
(4) Department of Ophthalmology, College of Medicine, Soonchunhyang University, Cheonan, Choongchungnam-do, South Korea |
| Jin Young Yang(1,2), Sanjar Madrakhimov(1,2), Ha Yan Park(2), Sang Joon Jung(3), Seung Kwan Nah(3), Tae Kwann Park(1,2,3,4) |
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목적 : To investigate the dynamics of mTOR-related protein levels in laser-induced choroidal neovascularization (CNV) model.
방법 : Laser photocoagulation (LP) was used to induce CNV in C57BL/6J mice. The time-course of CNV was observed by Fluorescein angiography (FAG) at 3, 5, 7 and 12 days after LP and analyzed for leakage area as well as intensity. The neuroretina and retinal pigment epithelium /choroid/sclera (RPE/Ch/S) complex were isolated for Western blotting (WB) analysis from CNV-induced mice eyes. mTORC1 and mTORC2 related proteins as well as other angiogenesis related proteins expression levels were detected.
결과 : FAG images confirmed the leakage from LP areas. FAG images analysis revealed the highest hyperfluorescence at 5 days. WB analysis demonstrated an increase in p-mTOR S2448 levels in lysates from both NR and RPE/Ch/S complex during the whole observation period, the results at 7 and 12 days were significant (P<0,05). The similar trend was observed in p-mTOR S2481 levels, however, significant upregulation was observed from 5 to 12 days (P<0,05). Corresponding to p-mTOR S2448 and S2481 levels, their downstream effectors levels were upregulated and found to be the significant at the same time-points
결론 : Our results clearly demonstrate that both mTORC1 and mTORC2 are involved in regulation of laser-induced CNV formation.
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