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목적 : Normal-tension glaucoma (NTG) is known as an optic neuropathy characterized by progressive retinal ganglion cell death and glaucomatous visual field loss. NTG is reported to be related with systemic hemodynamic factors, such as fluctuating blood pressure and systemic hypotension. However, the mechanism how these factors contribute to glaucomatous damage at the optic nerve head and retina is unknown. In this study, we investigated the role of cell death mechanism of the retina in NTG.
방법 : NTG induced to have systemic hypotension. Apoptosis of RGCs were examined by Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Expression of various markers related to RGC apoptosis and glial cell activation were analyzed by western blot analysis and immunohistochemical staining of the retina.
결과 : IOP elevation is not detected. We have analyzed the loss of Brn3a-positive RGCs and TUNEL-positive cells were detected in the ganglion cell layer. Expression of glial fibrillary acidic protein was increased throughout the retinal layer.
결론 : These findings suggest that systemic hemodynamic factors may contribute to the changes of astrocyte and muller cells in retina cell death without elevated IOP. The role of this phenomenon needs further investigation.
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