| 발표일자: |
2018년 11월 2일(금) ~ 11월 4(일) |
| 발표번호: |
P(e-poster)-325 |
| 발표장소: |
코엑스 컨퍼런스룸 3층 301 A-B |
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| 알칼리 각막화상에서 가교 콜라겐에 encapsulate된 중간엽줄기세포의 효과 |
| 1 가톨릭대학교 의과대학 안과학교실
2 가천대학교 화공생명공학화
3 Byers Eye Institute at Stanford University School of Medicine |
| 김진호(1), 이현종(2), 김현승(1), David Myung(3), 나경선(1) |
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목적 : We proposed that encapsulating hMSCs onto the ocular surface within collagen gels crosslinked with multifunctional PEG succinimidyl esters may be a useful way to deliver the secretome from immobilized mesenchymal stem cells (MSCs) to effectively suppress alkaline-burn induced corneal injury.
방법 : MSCs were added to neutralized collagen. Four-arm PEG-N-hydroxysuccinimide (NHS) was subsequently mixed to the desired concentration. Collagen gel rheology as a function of the concentration of 4arm PEG-NHS was analyzed to find the optimal cell density for encapsulation of MSCs. Subsequent to alkali-burn injury, MSCs encapsulated within crosslinked collagen gels were added to the wounded ex vivo rabbit cornea. The corneas were photographed and fixed for immunohistochemical evaluation. The controls group were collagen 4 arm PEG gels without cells, injury with no treatment, and no injury.
결과 : The degradation and rheological properties of different concentration of gels revealed the optimum concentration of 4-arm PEG NHS, collagen, and MSCs for sustained release of the secretome. All corneas in ex vivo organ culture lost their transparency immediately after alkali burn, and only the group treated with collagen-PEG-encapsulated MSCs recovered transparency after 10 days. Immunohistochemistry and confocal microscopy revealed the presence of α–smooth muscle actin (α-SMA) in the superior corneal stroma and epithelium layer of the no treatment group.
결론 : Encapsulating MSCs within collagen-PEG gels may be a promising platform for delivering their therapeutic benefits in cases of ocular inflammatory diseases such as alkali-burn injuries.
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