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목적 : To investigate differences between Korean and Caucasian eyes in major extracellular matrix (ECM) components of the lamina cribrosa (LC) and peripapillary sclera (PPS) and the epigenetic contribution to these racial differences.
방법 : Posterior segment tissues were obtained from 30 Caucasian donors and 42 age and axial length-matched Korean donors. Protein and mRNA expression of major ECM components were assessed. Methylation-specific PCR was performed to determine the percentage of methylated CpG sites in lysyl oxidase like-2 (LOXL2) genes. Histone modification was assessed by immunohistochemical staining for histone deacetylase (HDAC) 2 and HDAC3 and acetylated histone H3 in LC and PPS tissues.
결과 : Collagen and elastin were significantly more abundant in Korean eyes than Caucasian eyes. Expression of the ECM modulating enzyme LOXL2 was elevated in the PPS and LC of Korean eyes. Most of the CpG sites of the LOXL2 gene exhibited lower methylation in the LC and PPS in Korean eyes than Caucasian eyes. HDAC2 and HDAC3 expression levels were elevated in the nuclei of cells in the LC and PPS of Caucasian eyes.
결론 : DNA methylation and histone modifications influenced LOXL2 expression, resulting in racial differences in ECM composition of the LC and PPS.
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