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목적 : MicroRNAs (miRNAs) are noncoding RNAs that functions in silencing specific target mRNAs. The relative disease specificity of miRNAs and the accurate techniques for detecting these molecules have resulted in advancements in biomarker identification in the field of organ transplant. This study analyzes specific miRNAs expressed in corneal allograft rejection by using mouse corneal tissue.
방법 : Donor corneas were excised from C57BL/6 mice, which were sutured onto the recipient graft beds. As recipient grafts, BALB/c mice were used for allogeneic graft and C57BL/6 mice for syngeneic graft. At postoperative 4 weeks, corneal tissues were obtained from syngeneic control, rejected allograft graft, and accepted allograft graft. miRNA expression of each group was analyzed by using Affymetrix GeneChip® miRNA 4.0 Array. The experiment was repeated 3 times for confirming specific miRNAs expressed for each groups.
결과 : In microarray miRNA analysis, total of 1155 probes from control sample, 787 probes from accepted graft, 848 probes from rejected grafts were identified. 43 probes were up regulated and 179 probes down regulated in the accepted group when compared to the control (│Fold change (FC)│≥2, p<0.05). 44 probes were up regulated and 136 probes down regulated in the rejected group when compared to the control. 19 probes were up regulated and 66 probes down regulated in the accepted group when compared to the rejected. Certain miRNAs were commonly up regulated or down regulated in each group.
결론 : Significant differences in miRNA expression in syngeneic, accepted, and rejected grafts were noted. This shows that miRNA analysis in mouse corneal tissue may be a potent tool for predicting acute allograft rejection, and may be applicable in human sample studies.
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