| 외안F-040 |
| 자외선 유발 익상편 섬유아세포에서 재조합 인간 열쇽단백질 투여 효과 |
| ¹울산대학교 의과대학 서울아산병원 안과학교실
²아산생명과학연구소 |
| 문찬희¹, 신진아², 강순숙², 정혜린¹, 윤제환¹ 차흥원¹, 김재용¹ |
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목적 : To investigate the effect of recombinant human heat shock protein 27 (rhHSP27) on the ultraviolet irradiation induced human pterygium fibroblast.
방법 : Human pterygium fibroblast were isolated and cultured from specimen of normal conjunctiva and pterygium head, which collected after surgical excision. Cultured normal conjunctival fibroblasts were exposed to 20mJ/cm2 of UVB irradiation with and without 4 ug/mL of rhHSP27 treatments. Western blot analyses of α-smooth muscle actin (α-SMA), which is a marker for myofibroblast, were performed in each experimental groups and quantified using Image Gauge 4.0 software. The values of α-SMA / GAPDH were compared.
결과 : Pterygium head fibroblast showed 2.85 ± 0.11 fold greater α-SMA level compared to normal conjunctival fibroblast (P < 0.001). When normal conjunctival fibroblast exposed to UVB irradiation without rhHSP27 treatment, the level of α-SMA was increased by 1.92 ± 0.02 times (P < 0.001). However, when normal conjunctival fibroblast exposed to UVB irradiation with rhHSP27 treatment, the fibroblast demonstrated 0.82 ± 0.04 fold smaller α-SMA level than control (P=0.036).
결론 : The rhHSP27 has inhibitory effect on UVB induced differentiation of normal conjunctival fibroblast to pterygial myofibroblast. The rhHSP27 might be used as preventive treatment option for pterygium.
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