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목적 : To investigate the immunogenicity of Toxoplasma gondii infected dendritic cells derived exosomes (Toxo DC exo) with cholera toxin (CT) as adjuvant against T. gondii via mucosal routes including ocular and intranasal route.
방법 : BALB/c mice were immunized via mucosal routes including ocular and intranasal route with Toxo DC exo mixed with CT. Mice were immunized three times at 2-week intervals and we quantified serum (IgG) and mucosal (IgA) immunoglobulin from serum, tear, saliva, fecal, vaginal wash.
결과 : Mice vaccinated via intranasal route with Toxo DC exo+CT elicited significant higher humoral and mucosal immune responses in comparison with mice treated PBS, whereas the group immunized via ocular route with Toxo DC exo+CT induced significant T. gondii–specific Abs in only serum and tear and vaginal wash sample.
결론 : We demonstrated that ocular immunization with Toxo DC exo stimulate systemic and local antibody responses and propose that possibility of the ocular and intranasal route is an alternative to mucosal routes for T. gondii vaccines.
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