| 외안F-018 |
| 쥐에서 소포체 스트레스에의해 유발된 건성안에 대한 Rapamycin의 효과 |
| 한림대학교 의과대학 안과학교실(1), 한림대학교 의과대학 병리학교실(2), 분당서울대학교병원 안과학교실(3), 성균관대학교의과대학 안과학교실(4), 서울대학교병원 안과학교실(5). |
| 최상욱(1), 조범주(1), 김정원(2), 김주현(3), 정태영(4), 현준영(3,5), 위원량(5), 신영주(1) |
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목적 : To investigate whether rapamycin protect the tear and ocular surface against the endoplasmic reticulum (ER) stress-induced dry eye syndrome in mice.
방법 : Tunicamycin (TM) was injected intraperitoneally in balb/c mice with or without rapamycin. Phosphate buffered saline was injected intraperitoneally in control group. Blinking rate, fluorescein staining score (FSS) and phenol red-thread tear secretion test were measured 4 days, 1 week, and 2 weeks after treatment. Levels of inflammatory mediators and VEGF were measured by ELISA.
결과 : Blinking rate and FSS were elevated in TM group compared to control (p<0.05). Tear secretion was decreased in TM group compared to control (p<0.05). However, blinking rate, FSS and tear secretion were not different between rapamycin group and control group and were different between rapamycin group and TM group 4 days, 1 week, and 2 weeks after treatment (p<0.05). Levels of inflammatory mediators and VEGF were elevated in TM group and decreased in rapamycin group compared to TM group (p<0.05).
결론 : Intraperitoneal injection of TM induced dry eye syndrome in mice. Rapamycin protected the tear and ocular surface against the ER stress-induced dry eye syndrome in mice.
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