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목적 : Silica nanoparticles (SiNPs) are promising drug carriers for ophthalmic drug delivery. In this study we investigated the effect of various sizes of nonporous silica nanoparticles (SiNPs) on cultured human keratocytes.
방법 : Three different sizes of SiNPs (50, 100 and 150 nm) were manufactured. Primarily cultured human keratocytes were exposed to different concentrations (0, 25, 50, 100 µg/ml) of three sizes of SiNPs for up to 48 hours. Intracellular reactive oxygen species (ROS) generation, cellular viability, lactate dehydrogenase (LDH) assay, autophagy, vimentin expression and mTOR pathway activation were evaluated. Intracellular distribution of SiNPs were evaluated with transmission electron microscopy.
결과 : SiNPs were up-taken by keratocytes inside cytoplasmic vacuoles. Neither nuclear entry of SiNPs nor mitochondrial structural damages were observed. Both intracellular ROS generation and LDH level remained unchanged with up to 100ug/mL of SiNPs treatment. Cellular viability was not affected by SiNPs treatment. Autophagy showed significant dose dependent activation with 50 and 100 nm SiNPs. However, mTOR activation remained unchanged. Vimentin expression did not show any significant increase with SiNPs
결론 : Our findings suggested that 50, 100 and 150 nm sized SiNPs did not induce significant cytotoxicity in cultured human keratocytes at concentrations up to 100ug/mL
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