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목적 : Invasive methods such as histologic examination cannot be applied on longitudinal follow up. Therefore, non-invasive modalities are necessary for continuous evaluations in experiments. Funduscopy, Optical Coherence Tomography(OCT) and Electroretinography (ERG), are renown image devices for mice experiments to monitor pathological as well as functional changes of the ocular structures in vivo. However, there remained variations in the results because these diagnostic devices were not set up for the normative ranges and standard experimental methods
방법 : Standardized protocols for the accuracy using funduscopy, OCT and ERG, while setting up a normative range of cornea and retina thickness in BALB/C, C57BL6/J, 129s and C3H-KIBB mice. In order to establish the mice morphological experimental standard protocol, we have evaluated depth of anesthesia, dryness of cornea, temperature of body, and intensity of light exposure. OCT images of cornea and retina were taken from four strains of mice from 6-week-old to 16-week-old in every two consecutive week to evaluate the normative range.
결과 : In the comparison anesthesia depth between 80% and 120%, the lens opacity existed less in low depth of anesthesia. When cornea was wet enough, the lens opacity was lower. Intensity of light exposure might have increased the lens opacity. Cornea and retinal thickness were highly strain dependent. Differences between the cornea and retinal thickness of males and females have been observed only in a few cases. Cornea thickness widely varied in BALB/C than other strains. Fundus photo reflected in characteristic of each strain, there were no yellow spot and crack founded in C57BL6/J.
결론 : Taken together, it is important to standardize experimental protocol and establishing normative data for each strain for the achievement in the mouse phenotype consortium that tries to explore pathophysiology of various eye diseases through morphologic phenomenon of mice since they provide fundamental backgrounds for future experiments.
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