| 외안F-055 |
| 인간 군날개 섬유모세포에서 TGF-β2의 유도로 배양된 근섬유모세포에서 사이클로스포린의 항섬유화 효과 |
| 제일안과병원 제일안연구소¹
경북대학교 의학전문대학원 생리학교실²
경북대학교 자연과학대학 생물학과³ |
| 김연희1, 유성1, 배미정2, 정재창3, 이규원1, 하상원1, 박영정1 |
|
목적 : To investigate the effects of cyclosporine A (CsA) on fibrotic markers and extracellular matrix (ECM) in TGF-β2-induced myofibroblast on primary cultured human pterygium fibroblast cells
방법 : Primary cultured human pterygium fibroblasts subjected to experiment were exposed to 100 µg/mL of CsA for 20 minutes. Cells were washed with Dulbecco phosphate-buffered saline, and then incubated with TGF-β2 5ng/ml in serum-depleted Dulbecco modified Eagle medium/F-12 medium for 24 hours. Harvested cells were fixed and stained with fibrotic and ECM marker proteins. Expression levels of fibrotic and ECM marker proteins and the corresponding mRNA transcripts were determined by western blotting and real time reverse transcription polymerase chain reaction (RT-PCR) assays, respectively.
결과 : CsA reduces a number of myofibroblasts positive cells by TGF-β2-induced. CsA also markedly inhibited TGF-β2-induced α-smooth muscle actin (α-SMA) and ECM proteins on protein and mRNA expression level.
결론 : Our results demonstrate the anti-fibrotic effect of CsA in vitro. Further studies will be necessary to elucidate the precise intracellular signal mechanism responsible for CsA-induced down-regulation of myofibroblast by TGF-β2 induced on pterygium fibroblasts.
|
| |