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목적 : Aldehyde dehydrogenase 2 (ALDH 2) metabolizes acetaldehyde, the major cause of alcohol hangover symptoms. It also plays a vital role in the detoxification of reactive aldehydes. Age-related oxidative stress promotes lipid peroxidation of cellular membrane, leading to the accumulation of reactive aldehydes that cause corneal, lens, and retinal degeneration. Recently, a variety of anti-hangover products are commercially available, however, almost none of them has been proven to show enhanced metabolizing capacity of ALDH 2 in a live subject. We aimed to investigate a specific product of interest
방법 : The oxidizing capacity of the anti-hangover candy was examined by in vitro & in vivo experiments to measure the amount of NADH formation which is generated through catalytic conversion of alcohol and acetaldehyde, by using a spectrophotometer at 340 nm. Powder sample of a commercial anti-hangover product (KISLip®, Pico Entech, South Korea) was used as the experimental substance. In-vivo examination tested the ethanol and acetaldehyde concentration in blood of rats with oral infusion of experimental substance before or after ethanol intake. 50 SD male rats were randomly assigned into 5 groups.
결과 : In vitro measurements of the activities of alcohol dehydrogenase & aldehyde dehydrogenase within the anti-hangover substance were 1.84 unit/g and 0.28 unit/g, respectively. The oxidation capacities in experimental rats were dose-dependently increased after substance gavages. Particularly, the cases with oral intake of substance 220 mg/kg after 1hr of ethanol intake have shown more meaningful and obvious decreases in acetaldehyde concentration in blood through the period of all measurement times.
결론 : Oral intake of anti-hangover substance has significantly enhanced alcohol and acetaldehyde-metabolizing capacity in rat model, potentially suggesting increased ALDH2 capacity within circulation. Using this substance, further researches on animal model of age-related eye disease, including macular degeneration, and detoxification of other cytotoxic reactive aldehydes are recommended to conduct.
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