| 발표일자: |
2015년 11월 6일(금) ~ 11월 8일(일) |
| 발표번호: |
P(e-poster)-260 |
| 발표장소: |
킨텍스 제2전시장 7B홀 |
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| Microfluidic-based non-enzymatic glycation enhances cross-linking of human scleral tissue compared to conventional soaking |
| 경희대학교 의과대학 강동경희대학교병원 안과학교실1, 경희대학교 의과대학 경희의료원 안과학교실2, 경희대학교 의과대학 의공학교실3 |
| 김혜지1, 진경현2, 최삼진3, 신재호1 |
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목적 : The aim of this study was to evaluate nano-structural and chemical changes in human scleral collagen caused by non-enzymatic glycation using atomic force microscopy(AFM), Raman spectroscopy, and microfluidics.
방법 : Human scleral tissue were divided into 4 groups of pure sclera tissues (control group) and two scleral tissues groups (balanced salt solution[BSS]+DR7 group) and BSS+DR30 group) treated sequentially with incubation(1hr in BSS and D-ribose) and preservation for 7 and 30 days. Another scleral tissue(BSS+DR+μF7 group) group was incubated(1hr in BSS and D-ribose) and preserved for 7days in a microfluidic chip. After that, the surface of the fixed sclera tissues was scanned Atomic force microscopy (AFM) and Raman spectroscopy. One-way ANOVA was used to compare the mean values from each data set.
결과 : The scleral tissues incubated with BSS and D-ribose for 7 days in the microfluidic environment (BSS + DR + μF7) showed in a clear irregular parallel arrangement of collagen fibrils with tangled fibrils in AFM image. The Raman shift was observed at 919 cm–1; the BSS + DR30 group showed a shift of 2 cm–1 at 919 cm–1 whereas the BSS + DR + μF7 group showed a shift of 3.5 cm–1 at 919 cm–1. This Raman shift indicates the molecular changes in collagen.
결론 : Non-enzymatic glycation led to an increased in the density of both corneal and scleral stromal collagen. Our method using non-enzymatic glycation in a microfluidic environment successfully induced collagen cross-linking. These in vitro results suggested that glycation can be used to strengthen connective tissues.
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