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목적 : One serious ocular side effect associated with the long-term use of topical brimonidine tartrate is anterior uveitis. This study investigated the changes in the levels of several inflammatory cytokines, B-cells, and T-cells in rat eyes treated with topical brimonidine tartrate.
방법 : Twenty Sprague-Dawley male rats were divided into two groups of 10 rats each. In the brimonidine group, rats were treated with brimonidine three times per day for 10 months. The rat cytokine multiplex method was used to determine the levels of cytokines (IL-1α, IL-1β, IL-2, IL-6, TNF-α) in the conjunctiva, cornea, aqueous humor, and lens. The cornea and conjunctiva were subjected to immunohistochemical staining using anti-CD20 antibody and anti-CD3 antibody.
결과 : The concentrations of IL-1β, IL-2, and IL-6 in the conjunctiva were significantly lower in the brimonidine group.(P = 0.033, 0.017, 0.016)The concentration of IL-2 in the cornea was also significantly lower in the brimonidine group(P = 0.037). However, in the aqueous humor, the concentrations of IL-1β and IL-2 were significantly higher in the brimonidine group than in the control group(P = 0.016, 0.008).There was no significant difference in CD20+B cell and CD3+T cell infiltration of the conjunctival biopsy specimens.Corneal specimens also showed no infiltration of CD20+ B cell and CD3+Tcell
결론 : These results suggest that the increase in some inflammatory cytokines in the aqueous humor after long-term brimonidine treatment may contribute to the pathogenesis of brimonidine-induced uveitis.
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