| 외안F-025 |
| Increased Substance P Expression in the Ocular Surface and Trigeminal Ganglion in Murine Dry Eye Disease |
| 1Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School(1), Department of Ophthalmology, Hallym University Sacred Heart Hospital, Hallym University College of Medicine(2), Department of Ophthalmology and Visual Science, The Catholic University of Korea School of Medicine(3) |
| Sang-Mok Lee(1,2), Zahra Sadrai(1), Hyun Soo Lee(1,3), William Stevenson(1), Yihe Chen(1), Jing Hua(1), Brinda Subbarayal(1), Thomas Dohlman(1), Masahiro Omoto(1), Sunil Chauhan(1), Reza Dana(1) |
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목적 : Substance P is a key mediator of pain and neurogenic inflammation. In the present study, we analyzed protein and mRNA expression levels of substance P in the ocular surface and trigeminal ganglion (TG) to evaluate their association with the dry eye disease (DED).
방법 : DED was induced in 6-week-old female C57BL/6 mice by their placement in a controlled environmental chamber and subcutaneous scopolamine (25mg/kg) injection three times a day. Corneal fluorescent staining (CFS) was evaluated at days -1, 6, and 13, and corneas, conjunctivae, and TG were harvested at days 7 and 14 to analyze protein and mRNA expression levels of substance P. Substance P protein expression levels were measured with a competitive enzyme immunoassay kit and then adjusted to the total protein amount. Expression of substance P mRNA was analyzed by real-time PCR.
결과 : Substance P protein expression levels were increased in the cornea compared to the control group (Cornea: 1.15 fold on day 7, 1.86 fold on day 14, P<0.05). The mRNA expression of substance P was increased in both the cornea and TG (Cornea: 2.3 fold on day 7, 1.1 fold on day 14, P<0.05; TG: 1.5 fold on day 7, 1.3 fold on day 14, P<0.05). Increased protein expression of substance P in the cornea correlated with increased CFS scores (r = 0.900, P<0.001).
결론 : Protein and mRNA expression levels of substance P are increased in ocular surface and TG in DED but in a different manner. Increased expression of substance P in the cornea correlated with increased CFS scores.
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