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목적 : To investigate whether an anti-inflammatory protein TSG-6 (TNF-α stimulated gene/protein 6) might have protective effects on corneal endothelial cells in rabbits with transcorneal freezing injury.
방법 : Transcorneal freezing (-80°C) was applied to the corneal surface for 30 sec in New Zealand White rabbits. Right after injury, TSG-6 (10 μg/100 μl), STC-1 (10 μg/100 μl), or the same same volume of BSS was injected into the anterior chamber. Corneas were examined for opacity every 6 hours during 48 hours post-injury. Corneal thickness, endothelial cell counts, and hexagonality of endothelial cells were also measured. At 48 h post-injury, the animals were humanely killed, and the corneas were subjected to analyses.
결과 : TSG-6 significantly decreased development of corneal edema, compared to BSS- or STC-1. Tthe number of corneal endothelial cells and hexagonality were markedly preserved by TSG-6. However, there were no differences in corneal thickness and endothelial cell counts between BSS- and STC-1-treated eyes. Vital staining showed that there were fewer number of dead cells in corneal endothelium of TSG-6-treated eyes. Molecular assays showed that the levels of pro-inflammatory cytokines in the cornea and aqueous humor were significantly reduced by TSG-6.
결론 : TSG-6 protected corneal endothelial cells from transcorneal freezing injury by suppressing inflammation.
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