| 외안F-011 |
| Protection from Hemolytic Uremic Syndrome by Eyedrop Vaccination with Modified Enterohemorrhagic E. coli Outer Membrane Vesicles |
| 국민건강보험공단 일산병원(1),한국생명공학연구원(2),연세대학교 의과대학 안과학교실(3) |
| 최경섭(1),김상현(2),김은도(3),서경률(3) |
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목적 : We investigated whether eyedrop vaccination using modified outer membrane vesicles (mOMVs) is effective for protecting against hemolytic uremic syndrome (HUS) caused by enterohemorrhagic E. coli (EHEC) O157:H7 infection.
방법 : Modified OMVs and waaJ-mOMVs were prepared by culturing MsbB- and STxA-deficient EHEC O157:H7 bacteria with or without an additional waaJ mutation. BALB/c mice were immunized by eyedrop mOMVs, waaJ-mOMVs, and mOMVs plus polymyxin B (PMB). Mice were boosted at 2 weeks, and challenged peritoneally with wild-type OMVs (wtOMVs) at 4 weeks. We quantified serum and mucosal immunoglobulins, and body weight and blood BUN/Cr were monitored after challenge. We observed pathological changes in renal tissue.
결과 : The group vaccinated with mOMVs elicited a greater humoral and mucosal immune responses than did the waaJ-mOMVs and PBS-treated groups. Eyedrop vaccination of mOMVs plus PMB elicited reduced humoral and mucosal immune responses. In the challenge test, mice vaccinated with mOMVs were protected from a lethal dose of wtOMVs administered intraperitoneally.There were no abnormal body weight changes or histopathological findings in the ocular tissue after eyedrop vaccination of mOMVs.
결론 : In conclusion, mOMV administered by eyedrop may be utilized as an efficient and safe vaccine against EHEC O157:H7 infection and resulting HUS.
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