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목적 : To investigate neuroprotective and regenerative properties of transforming growth factor-Beta (TGF-β) 3 in mouse retinal ganglion cell (RGC) after optic nerve crushed.
방법 : The mice (C57BL/6J) were anesthetized and the optic nerves of mice were crushed. With micro-forceps, grasp the edge of the conjunctiva next to the globe and retract it, rotating the globe nasally. The exposed optic nerve is grasped approximately 1–3 mm from the globe with forcep for 10 s. Intravitreal injections of TGF- β3 (20 ng/ml) were performed 1 day after crush. For the analysis of ganglion cell survival, the mice were allowed to survive for 1 day, 3 days, 7 days and 21 days, after which they were harvested and ganglion cell survival was assessed.
결과 : A significant loss of fluorescent spots was found after PBS injected 1, 3, 7 and 21 days with 71.24±1.53%, 66.16±1.8%, 54.70±2.1% and 49.95±1.17% of RGC remaining (counterstained with DAPI) at the group of crush only compared to normal control (P <0.005 ; n = 6). Intravitreal injection of TGF-β3 protected RGC loss in 3 days after optic nerve injury (P < 0.02). At 21 days, RGC cell numbers were significantly increased in TGF-β3 injection group (P < 0.05). Moreover, degradation of nerve fiber (NF) was markedly protected in TGF-β3 injection group compare to crushed and PBS-injected groups.
결론 : After nerve crush, RGCs died rapidly, but intravitreal injection of TGF-β3 has effect of neuroprotection and regeneration in RGC and NF.
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