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목적 : To investigate whether PGs would affect wound healing and eventual fibrotic encapsulation after implantation of a glaucoma drainage device in a rabbit
방법 : New Zealand White rabbits underwent Ahmed glaucoma valve implantation and randomly received topical ocular administration of travoprost (PG) or vehicle solution once per day. Histopathologic examination of the bleb tissue and immunostaining for proliferating-cell-nuclear-antigen (PCNA), matrix metalloproteinases(MMP), tissue inhibitors of metalloproteinase (TIMP), connective tissue growth factor (CTGF), antiactin, α–smooth muscle (α-SMA) were performed.
결과 : Hematoxylin-and-eosin-stained sections revealed that cellularity in the bleb tissue was greater in the travoprost group than the control group. The proportion of PCNA–positive cells was greater in the travoprost group (P=0.010). In travoprost-treated eyes, immunofluorescence staining for MMP was increased, but that for TIMP immunoreactivity was lower than in the control eyes. Stimulation of CTGF by travoprost was observed in the bleb tissue. The extent of α–SMA immunostaining was greater in the travoprost group than the control group. By Masson’s trichrome staining, the inner collagenous layer was thicker in the travoprost group (P=0.008).
결론 : Promotion of fibrosis by the PG analogue seemed to occur via stimulation of fibroblast proliferation and migration and by production of CTGF. Frequent development of the hypertensive phase after glaucoma drainage device implantation can lead to use of antiglaucoma eyedrops in the early postoperative period. Our findings suggest caution in the use of PGF2α analogues early after glaucoma surgery and modulation of them might be a new therapeutic target for reduction of fibrosis.
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