| 발표일자: |
2014년 4월 12일(토) ~ 4월 13일(일) |
| 발표번호: |
P(e-poster)-078 |
| 발표장소: |
킨텍스 제2전시장 7B홀 |
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| Neonatal immune challenge alters retinal vessel development in rats. A potential novel model for retinopathy of prematurity |
| 서울대학교 의과대학 안과학교실1, 분당서울대학교병원 안과2, 분당서울대학교병원 소아청소년과3, 서울대학교병원 안과4 |
| 우세준1,2, 홍혜경2, 박규형1,2, 최창원3, 오주연1,4 |
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목적 : Inflammation is being highlighted as the major pathogenic factor for the morbidity of prematurity. We intended to investigate the effect of neonatal systemic inflammation on the retinal vascular development in newborn rats and to show the role of inflammation in the retinopathy of prematurity.
방법 : Systemic inflammation was induced by the pre- and post-natal intraperitoneal injections of lipopolysaccharide (LPS) in neonatal rats. Enucleation and retinal separation was performed at 1 and 2 post-natal weeks. Analyses on morphology of retinal vessels, recruited immune cells, and quantification of inflammatory cytokines were assessed with immunofluorescence, FACS, and RT-PCR.
결과 : Administration of LPS postnatlly, not prenatally, induced inflammation in the retina with recruitment of CD11c positive cells and increased expression of a set of inflammatory cytokine genes. Postnatal LPS administration also suppressed retinal vessel development and deranged the retinal vascular structures combined with abnormal vascular tuft formation that resembles features of retinopathy of prematurity. Retinal infiltration of cd11c positive cells was colocalized with focal sprouting of astrocytes and excess angiogenesis. Increased apoptosis of inner retinal cells was also observed.
결론 : Neonatal systemic inflammation alters the normal development of retinal vessels by inducing retinal inflammation. Our model of systemic inflammation-induced abnormal development of retinal vasculature can be served as a new experimental model of retinopathy of prematurity.
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