| 발표일자: |
2014년 4월 12일(토) ~ 4월 13일(일) |
| 발표번호: |
P(e-poster)-236 |
| 발표장소: |
킨텍스 제2전시장 7B홀 |
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| Experimental Dry Eye-associated Neuroinflammatory Changes in Trigeminal Ganglia |
| 1.가톨릭대학교 안과 및 시과학교실
2.Schepens Eye Research Institute and Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston, Massachusetts |
| 이현수, Kishore Reddy, Sunil K. Chauhan, 이상목, 주천기, Reza Dana |
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목적 : Dry eye disease (DED) is one of the most common ophthalmic pathologies, affecting approximately 10 million people in the United States alone. Recently, it has become recognized that DED is associated with ocular surface inflammation and morphological changes in corneal nerve axons (extension of the trigeminal nerve). To determine whether DED induces neuroinflammatory changes in trigeminal ganglion (TG), we evaluated the expression of proinflammatory cytokines and neuropeptides in TG in a murine model of DED.
방법 : 7-8 week old female C57BL/6 mice underwent main lacrimal gland excision, and were housed in a controlled environment chamber to induce experimental DED. Mice housed in room air following surgery served as controls. Expression of proinflammatory cytokines and neuropeptides in the cornea or TG were evaluated using real-time polymerase chain reaction (PCR). In addition, glial fibrillary acidic protein (GFAP) expression and immune cell infiltration of TG were assessed by real-time PCR and flow cytometry.
결과 : DED significantly increased mRNA expression of IL-6, TNF, and substance P in cornea and TG at day 5 and day 14, and CGRP and NGF mRNA expression increased in cornea but not TG. In the DED group, TG showed increased expression of GFAP and infiltration of CD11b+ cells.
결론 : In conclusion, these novel findings suggest that DED-induced neuroinflammatory changes occur at the level of the trigeminal ganglion, and are not only restricted to the ocular surface.
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