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목적 : Purpose: Herein we demonstrate that iPS from human dermal fibroblast can be induced to differentiate towards limbal progenitor cells.
방법 : Human dermal fibroblast cells were reprogrammed using a doxycycline inducible
lentiviral vector containing Klf4, Sox2, Oct4, and c-myc in culture using an radiation-treated mouse embryonic fibroblast (MEF) feeder layer in ES culture medium. Limbal progenitor cells from these iPS cells by co-culture with PA6 feeder cells on limbal specific media the BMP4 and WNT treatment. Differentiated cells were analyzed by PCR and immunocytochemistry and cultured in the transwell with corneal epithelial medium.
결과 : Human dermal fibroblast-derived iPS cells are able to give rise to the limbal
progenitor cells in vitro. Moreover, we obtained multilayered corneal epithelial cells from these limbal progenitor cells by air-lift culture assay.
결론 : The present study demonstrate the successful reprogramming strategy for
limbal progenitor cells differentiation from human dermal fibroblast derived iPSC using a mix of 4 factors and further suggests the therapeutic application of limbal progenitor cells from iPS cells for the limbal stem cell deficiency in the future.
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