| 발표일자: |
2014년 4월 12일(토) ~ 4월 13일(일) |
| 발표번호: |
P(e-poster)-039 |
| 발표장소: |
킨텍스 제2전시장 7B홀 |
|
|
| Sulodexide inhibits retinal neovascularization in a mouse model of oxygen-induced retinopathy |
| Department of Ophthalmology, Incheon St. Mary’s Hospital,College of Medicine, The Catholic University of Korea, Incheon, Korea (1)
Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, Gangneung, Korea (2)
|
| Jae Wook Han(1), Sang Hoon Jung(2), Hyungbin Hwang(1), Na Young Lee(1), Hye Bin Yim(1), Kui Dong Kang(1) |
|
목적 : The aim of this study is to investigate whether sulodexide is effective in inhibiting retinal neovascularization in an oxygen induced retinopathy (OIR) mouse model.
방법 : ICR neonatal mice were exposed to 75% oxygen from postnatal day (P)7 until P12 and returned to room air (21% oxygen) for five days (P12 to P17). Mice were subjected to daily intraperitoneal injection of sulodexide (5 mg/kg, 15 mg/kg) or saline from P12 to P17. Retro-orbital injection of FITC-dextran was performed and retinal flat mounts were viewed by fluorescence microscopy and photographed. Avascular area and neovascular tufts were quantified from the digital images in a masked fashion using image analysis software (SWIFT_NV). Neovascular nuclei were also counted in the in OIR induced mouse (P17) and sulodexide-injected mouse. Western blot was performed to characterize the angiogenic processes involved in OIR and to demonstrate the anti-angiogenic activity of sulodexide in vivo.
결과 : The retina of the OIR induced mouse (P17) had a distinctive area of central nonperfusion. In the retina of sulodexide-injected mouse (15mg/kg), this central ischemic area was significantly decreased (47.87±4.51% vs 36.25±2.96%, P<0.01). The mean numbers of neovascular nuclei counted in OIR induced mouse (P17) and sulodexide-injected mouse (15 mg/kg) were significantly different in HE staining(p<0.01). Hyperbaric oxygen exposure resulted in an increase of VEGF, MMP-2, MMP-9 and when mice were treated with sulodexide, a dose dependent reduction in VEGF, MMP-2, MMP-9 was observed. Both high do
결론 : Our study demonstrates the anti-angiogenic effect of sulodexide in a mouse model of OIR in vivo. Our result suggest that sulodexide can be one of the candidate supplementary substances to be used for the treatment of various ocular vascular pathologies such as retinopathy of prematurity or diabetic retinopathy.
|
| |