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목적 : Purpose: Trying to analyze the differences in the structure of collagen between a human preserved amniotic membrane (gAM), a human placental amniotic membrane (hAM), a human reflected amniotic membrane (rAM) and corneoscleral tissue by using Raman spectroscopy and histological analysis.
방법 : Methods: Three AM biomaterials (n=4 for each type) including pAM group, rAM gorup, and gAM group were used. Four human corneo-scleral tissues were collected at the Eye Bank of the Kyung Hee University Medical Center. The H&E and MT images were captured by an Olympus BX51 microscope equipped with a CCD camera. Density-based analysis was performed using ImageJ software. A SENTERRA confocal Raman system equipped with a 100 mW, 785 nm diode laser spurce and a 3–5 cm-1 resolution was used to acquire the Raman spectra.
Data were analyzed using Matlab computing software.
결과 : Results: AM groups showed a significant decreased collagen distribution compared to two EYE groups (p < 0.001). Particularly, pAM group showed significantly lower collagen density than rAM. However, we could obtain no histological information from gAM group. In the analysis of Raman spectroscopy the amide I (1655~1667 cm-1) and amide III (1241~1276 cm-1) bands, which indicate the stabilization of a subfibrillar triple helical structure of collagen showed significant low peak in gAM group.
결론 : Conclusion: The preserved amniotic membrane has a weaker collagen structure than other groups. It is believed that preserved amniotic membrane has a weak tensile strength and drops out early in clinical use.
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