| 발표일자: |
2014년 4월 12일(토) ~ 4월 13일(일) |
| 발표번호: |
P(판넬)-081 |
| 발표장소: |
킨텍스 제2전시장 7B홀 |
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| Human Umbilical Cord Blood cells and Chorionic Plate derived Mesenchymal Stem Cells Promote Retina Ganglion Cell Survival in SD-Rat Models with Traumatic Optic Neuropathies |
| (1) Department of Ophthalmology,
(2) Department of Biomedical Science,
(3) Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Gyeonggi-do, Korea;
(4) CHA Medical Center Cord Blood Bank, Gyeonggi-do, Korea |
| HyungChul Kim(1), SeokJoong Chung(1), SeungSoo Rho(1), GiJin Kim(2), KwangHyun Baik(2) MyungSeo Kang(3,4), Helen Lew(1) |
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목적 : Even though ongoing research is dedicated to the management of traumatic optic nerve injury with various measures, further strategies of treatment based on the complex underlying major mechanism such as inflammation and/or ischemia is required. We therefore investigated compressed rat optic nerve following transplantation with human chorionic plate-derived MSCs (CP-MSCs) isolated from placenta and human UCB.
방법 : After making rat models of optic nerve crush, CP-MSCs, and UCB stem cell injected into left internal carotid artery for each subject. To define the stem cell response, animals were euthanized 1, 2, 4 weeks after injection. The eyeballs were enucleated to assess the axon survival ratio and immunohistochemical evaluation of normal right side and ONC left side in each rat. The expression markers for inflammation, apoptosis and optic nerve regeneration were analyzed with axon survival rate by direct counting.
결과 : Whereas IL-2 activities and GAP43 were increased (p < .05), the expression levels of hypoxia-inducible factor-1a (HIF-1a) were significantly increased by UBC injection than CP-MSCs injection. Increased axon survival rates (p < .005) were observed in UBC injection and CP-MSCs injection compared to controls. Furthermore, the upregulation of HIF-1a promotes the survival of damaged axons through recovery from hypoxia in UBC injection than CP-MSCs injection.
결론 : These results suggest that the administration of UCB promotes axon survivals by systemical concomitant mechanisms involving HIF-1a. These findings provide further understanding of the mechanisms involved in traumatic optic nerve injury and will help develop new cell based therapeutic strategies for regenerative medicine in optic nerve disease
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