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목적 : To evaluate and compare the role of astrocytes and lamina cribrosa (LC) cells in the modulation of the extracellular matrix (ECM) of the optic nerve head (ONH).
방법 : Astrocytes and LC cells were cultured from the LC of Asian donor ONHs and treated with 1.0, 5.0, and 10.0 ng/mL concentration TGF-β2 for 24 hours. Astrocytes were differentiated from LC cells through the use of a primary antibody against glial fibrillary acidic protein (GFAP), which is expressed by ONH astrocytes, but not by LC cells. Total cellular protein was extracted from the astrocytes and LC cells and the blots were incubated with collagen I (COLI), collagen III (COLIII), fibronectin (FN), connective tissue growth factor (CTGF), and beta-actin antibodies in blocking solution.
결과 : TGF-β2 treatment increased cell numbers and caused cell hypertrophy in both astrocytes and LC cells. An increase in TGF-β2 concentration produced an incremental increase in the amount of GFAP in astrocytes. After TGF-β2 treatment, an increase in the amount of COLI was found in both astrocytes (100%) and LC cells (50%). However, COLIII did not increase in either cell type. FN increased in both cell types after TGF-β2 treatment, with astrocytes demonstrating a larger increase.
결론 : In cultured human ONH LC glial cells, there was a notable change in the ECM following stimulation with TGF-β2; the change in the ECM was greater in astrocytes than in
LC cells.
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