| 녹F-039 |
| The Combined Injection of Transforming Growth Factor-Beta (TGF-β) and Staurosporin has Neuroprotective Effects in a Mouse Optic Nerve Crush Model |
| 제일안과병원 제일안연구소(1), 경북대학교 자연과학대학 생물학과 |
| 이태윤1, 김연희1, 김은주1, 구성현1, 정재창2, 강선희2, 이세형2, 박영정1, 이규원1 |
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목적 : To determine whether transforming growth factor-Beta (TGF-β) and staurosporin (STS) have neuroprotective activity on mouse retinal ganglion cell (RGC) after optic nerve crushed.
방법 : The mice (C57BL/6) were anesthetized. The optic nerves of mice were crushed. It was crushed with micro-forceps by grasping the edge of the conjunctiva next to the globe and retracting it, and rotating the globe nasally. The exposed optic nerve was grasped approximately 1–3 mm from the globe with forceps for 10 s. Intravitreal injections of TGF-beta (20 ng/ml) and STS (500 µM) 2 µl were performed 1 day after the crush. For the analysis of ganglion cell loss, the mice were allowed to survive for 30 days, after which they were harvested and ganglion cell survival was assessed.
결과 : A significant and marked loss of fluorescent spots was found after optic nerve crush with 36.37±2.3%, 55.89±8.6%, and 66.20±3.1% of DAPI expression RGC remaining at groups of crush only, TGF-β2+STS and TGF-β3+STS, respectively, compared to normal control (P < 0.002 ; n = 5). Intravitreal injection of TGF-β3 and STS combination protected RGC loss in 30 days after optic nerve injury (P < 0.02).
결론 : After nerve crush, RGCs died rapidly, but intravitreal injection of TGF-β and STS had effect of neuroprotection in RGC. Our data suggest that injection of TGF-β and STS into the vitreous may have a potential therapeutic effect in retinal diseases.
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