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목적 : To investigate the phenotype of macrophages infiltrating corneal allografts in acute or chronic rejection in mice and humans.
방법 : We performed corneal allotransplantation in mice and sacrificed animals on day 28 during acute rejection when 70% of corneal allografts were rejected, and divided the grafts into two groups - grafts with rejection as rejectors and grafts without rejection as non-rejectors, and analyzed for macrophage infiltration and their phenotype, M1 or M2 type macrophages. In addition, we assayed for M1 or M2 macrophage- specific cytokines and markers in corneal allografts with chronic rejection in humans.
결과 : We found that a large number of iNOS+ F4/80+ cells representing M1 macrophages were accumulated in rejected corneal allografts in mice. In contrast, many MRC (mannose receptor)+ cells indicating M2 macrophages were present in non-rejectors while there were rare MRC+ cells in rejectors. Levels of IL-1β, IL-12a, iNOS, and CCL3 that are produced by M1 macrophages were highly increased in rejectors, while levels of MRC1, MRC2, and IL-10 that are expressed by M2 macrophages were higher in non-rejectors. Human corneal grafts with chronic rejection showed similar pattern.
결론 : Large numbers of M1 macrophages infiltrated rejected corneal allografts in mice and humans, implicating these cells in the pathogenesis of corneal allograft rejection.
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