| 발표일자: |
2013년 11월 1일(금) ~ 11월 3일(일) |
| 발표번호: |
P(판넬)-045 |
| 발표장소: |
킨텍스 제2전시장 7B홀 |
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| 당뇨 망막병증과 연관된 염증성 사이토카인과 소포체 스트레스 발생에서의 아연과 아연 이온운반체인 clioquinol의 역할 |
| 서울아산병원 울산대학교 의과대학 안과학교실(1), 신경과학교실(2), 의생명연구소 뇌신경연구단(3) |
| 정유리(1), 최정아(3), 고재영(2, 3), 윤영희(1) |
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목적 : The early event of diabetic retinopathy (DR) is the breakdown of blood-retinal barrier, leading to the increased secretion of inflammatory cytokines. We investigated the role of endoplasmic reticulum (ER) stress in inflammatory cytokines production, and verified the protective effect of clioquinol and zinc in human retinal pericytes (HRP) treated with advanced glycation end products (AGE) or modified low-density lipoprotein (mLDL).
방법 : Cultured HRP cells were treated with 500 ug/ml AGE or 100 ug/ml mLDL, for 24 hours. Clioquinol, the zinc ionophore, was added with or without zinc supplementation. The expression of GRP78 and MCP-1 was determined by western blot, for the evaluation of the ER stress and the induction of diabetes-related inflammatory cytokines respectively.
결과 : After exposure of 500 ug/ml AGE or 100 ug/ml mLDL, GRP78 was increased at 6 and 12 hours, respectively. The expression of MCP-1 was increased in 16 hours of exposure. The expression of GRP78 and MCP-1 was attenuated by clioquinol and zinc supplementation in AGE or mLDL-exposed HRP.
결론 : We verified that the expression of GRP78 and MCP-1 was increased in AGE or mLDL-exposed HRP, and that clioquinol attenuated this with zinc supplementation. This suggests that clioquinol may prevent ER stress and the induction of diabetes-related inflammatory cytokines by restoring intracellular zinc level, and further evaluation is needed for its possible protective effect in diabetic retinopathy.
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