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목적 : To investigate the effect of chondrocyte-derived extracellular matrix (CDECM) on an experimental corneal alkaline burn in rabbit.
방법 : Corneal neovascularization (NV) was induced by 1N NaOH soaked 8 mm filter paper to the right central cornea for one minutes in rabbits. Ten days later, the rabbits were randomly divided into three groups; alkaline burns group; CDECM transplantation group; human amniotic membrane (HAM) transplantation group. The left eyes were used as control. CD-ECM and HAM were transplanted onto the corneal surface to completely cover the resected area and sutured. On 10 days after transplantation, a histological analysis evaluated the structure change of the cornea. We examined that the effect CDECM on clinical NV features and on expression of corneal NV markers.
결과 : Alkaline burn produced significantly NV and thickness in the cornea. On day 10 after transplantation, the thickness, NV and opacity of cornea were markedly decreased by the CDECM group (p<0.001). However, the HAM transplantation was not improved these clinical parameters, as well as the level was not significantly different with the burn group. In addition, the CDECM was improved alkaline burn-induced the cornea appeared disruption of the epithelium, proliferation and fibrotic changes of the stroma. The hallmarkers of NV such as CD31, VEGF, bFGF and MMP-9 were significantly expressed in the subepithelium by alkaline burn, whereas it’s levels were also suppressed by CDECM. The suppression mechanism of CDECM on corneal NV resulted from inhibition of NFκB activation through blocking of PKC and Akt signaling pathway.
결론 : The CDECM transplantation markedly effective in healing alkali-burned corneas through modulated the translocation of NFkB to nuclear. It could be used as a promising material for the noninvasive treatment of ocular surface disease. (This study was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry of Health and Welfare Affairs, Republic of Korea; grant #: HI12C0005 and HI12C0054)
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